| Acetonitrile is perhaps the
most popular solvent in modern molecular biology.
In peptide synthesis it is used to dissolve the
phosphoramidite precursors prior to manual or
automated synthesis. For this application, the
acetonitrile must be as dry as possible since
residual water has an adverse effect on the
maximum chain length that can be achieved. ROMIL manufacture several
special grades of anhydrous acetonitrile suitable
for all such techniques. Water levels in the
driest quality ’10 BiO’ are typically
around 5ppm (0.0005%). Packaged in sealed vials
it retains the maximum dryness until point of use.
For less demanding
applications such as washing through of
reactants, it is available in lower cost grades
such as ’30 BiO’ and ’50 BiO’.
The product description denotes the maximum level
to which residual water is controlled.
Each pack of ROMIL
Acetonitrile BiO is provided with an actual lot
analysis printed on the label of residual water
content determined by coulometric Karl Fischer
titration.
Both the 1LT and 2½LT
bottles feature a GL/DIN/ISO 45E thread and a
special adaptor is available (ROMIL Code PAD45E)
to enable direct connection to automated
synthesisers.
Furthermore, to
facilitate the handling of large volumes in
commercial production of peptides and genome
sequencing, we have available larger stainless
steel flasks, under the LiQuiSure™ brand
which can be pressurised for automated and
continuous dispensing of acetonitrile as well as
other solvents and liquid reagents. When one drum
is emptied a special control unit switches to a
full drum without any interruption in supply. We
are very proud of this system which has been
designed by ROMIL scientists and is proven in use
through years of trouble-free operation at
customer locations. For further information ask
us for details of LiQuiSure™ Equipment and
Accessories or click here to download a PDF file of
this section of our printed catalogue (531 KB) .
|
ROMIL-BiO™ is a range
of solvents of particular interest to molecular
biologists and biochemists. Purification methods,
although derived from those used for